Can resorufin be used in inverse fluorescence assay to detect H2O2?
What is the absorption band of resorufin 90 in THF?
- Resorufin based chemodosimeter 90 (Chart 16) in THF [145] showed absorption bands at 347 and 437 nm which disappeared with emergence of new absorption bands at 550 and 591 nm in the presence of TBAF with a reaction time of 9 min and displayed fluorescence emission enhancement centred at 595 nm. Fiona A. Summers, ...
How do you measure resorufin with spectrophotometer?
- After adding the reaction mixture, resorufin is measured every 30 s for 1 h using a spectrophotometer at 560 nm (Spectramax Plus 384; Molecular Devices, Sunnyvale, CA). Alternatively, resorufin can be measured using a fluorescent plate reader at 560 nm excitation and 590 nm emission detection.
What makes resorufin a good platform for biological sensors?
- Luke D. Lavis, in Progress in Molecular Biology and Translational Science, 2013 The long wavelength of resorufin makes it an attractive platform for sensors. One example is the reduced boronic ester 74, which reacts spontaneously with H 2 O 2 to yield the fluorescent resorufin.
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